5067-5589 [HP]
Agilent D5000 ScreenTape and High Sensitivity D5000 ScreenTape Assays;
| 型号： | 5067-5589 |
| 厂家： | 
HEWLETT-PACKARD |
| 描述： | Agilent D5000 ScreenTape and High Sensitivity D5000 ScreenTape Assays |
| 文件： | 总2页 (文件大小：432K) |
| 中文： | 中文翻译 | 下载： | 下载PDF数据表文档文件 |
Agilent D5000 ScreenTape and High
Sensitivity D5000 ScreenTape Assays
Accelerate DNA analysis with the Agilent
4200 TapeStation system
Data Sheet
Fast and easy DNA quality
control
The Agilent 4200 TapeStation system
offers automated sample processing of
1 to 96 samples for quick and reliable
RNA and DNA sample quality control.
The D5000 ScreenTape assay allows
for the separation and analysis of DNA
fragments from 100 bp to 5,000 bp,
ideal for larger sized NGS libraries.
Depending on the sample concentra-
tion, it is possible to choose between
the D5000 ScreenTape and the
High Sensitivity D5000 ScreenTape
application.
Easy to use
Sample quality control analysis has
never been so easy – simply load the
4200 TapeStation system with the
appropriate D5000 ScreenTape device,
loading tips, and samples in tube strips
or 96-well plates, and you will be review-
ing results in less than 2 minutes per
sample.
Simplify your sample quality control
Complete solution for DNA
analysis
• Agilent 4200 TapeStation system
(G2991AA)
• Agilent D5000 ScreenTape Assay
* D5000 ScreenTape (5067-5588)
* D5000 Reagents (5067-5589)
* D5000 Ladder (5067-5590)
• Agilent High Sensitivity D5000
ScreenTape Assay
with ready-to-use ScreenTape con-
sumables and reagents.
Scalable throughput
Analyze any number of samples at con-
stant price per sample.
Fast results
Obtain results in as little as one min-
ute per sample independent of total
sample number.
Key Features
Excellent accuracy and
precision
Reliably analyze size and quantity of
PCR fragments, fragmented DNA, and
DNA libraries.
Zero carryover
* High Sensitivity D5000
ScreenTape (5067-5592)
* High Sensitivity D5000
Reagents (5067-5593)
* High Sensitivity D5000 Ladder
(5067-5594)
Avoid sample carry over by running each
DNA sample in a separate lane of the
ScreenTape device.
Minimal sample consumption
Use no more than 2 μL of your pre-
cious samples per run – even for high
sensitivity analysis.
High sensitivity
Qualify your precious DNA sample
down to a concentration of 5 pg/μL
per fragment.
Agilent D5000 ScreenTape applications
DNA analysis during the NGS
workflow
The Agilent 4200 TapeStation system
and the DNA ScreenTape assays can be
used at several steps of the Next gener-
ation Sequencing (NGS) workflow such
as shearing of genomic DNA and library
amplification to ensure DNA library and
sample quality. The figure shows the
analysis of sheared genomic DNA for an
NGS library preparation workflow with
the D5000 ScreenTape assay.
Analytical
Specifications
Agilent
D5000 ScreenTape Assay
Agilent High Sensitivity
D5000 ScreenTape Assay
Sizing range
100 bp – 5,000 bp
400 – 5,000 bp: 15%
0.1 ng/µL
100 bp – 5,000 bp
400 – 5,000 bp: 15%
5 pg/µL
Typical resolution
Sensitivity 1
Sizing precision 2
Sizing accuracy 2
Quantitative precision
5% CV
10% CV
± 10%
± 15%
0.1 – 1 ng/µL: 15% CV
1 – 50 ng/µL: 10% CV
15% CV
Quantitative accuracy 3
Quantitative range
± 20%
± 25%
0.1 – 50 ng/µL
10 – 1,000 pg/µL
Maximum sample buffer
strength
250 mM KCl
250 mM Tris-HCl
125 mM NaCl
50 mM Acetate
25 mM MgCl2
25 mM BSA
25 mM KCl
25 mM Tris-HCl
12.5 mM NaCl
5 mM Acetate
2.5 mM MgCl2
2.5 mM BSA
25 mM Guanidine-HCl
2.5 mM Guanidine-HCl
Learn more
1 Signal/noise ratio > 3 for a single peak
2 Determined using D5000/High Sensitivity D5000 ladder as sample
www.agilent.com/genomics/tapestation
3
Measured against 2200 TapeStation system
Buy online
www.agilent.com/chem/store
Physical
Specifications
Agilent
D5000 ScreenTape Assay
Agilent High Sensitivity
D5000 ScreenTape Assay
Find an Agilent customer center
www.agilent.com/chem/contactus
Analysis time
15 samples < 25 minutes
96 samples < 135 minutes
15 samples < 20 minutes
96 samples < 120 minutes
Samples per consumable
Sample volume required
Kit stability
15
15
For Research Use Only.
1 µL
2 µL
Not for use in diagnostic procedures.
4 months
105 samples
4 months
105 samples
This information is subject to change without
notice.
Kit size
© Agilent Technologies, Inc., 2015, 2017
Published in USA, June 1, 2017
Publication Number 5991-5211EN
相关型号：
©2020 ICPDF网 联系我们和版权申明