5067-4627 [HP]
Kits and Reagents for the Agilent 2100 Bioanalyzer System;型号: | 5067-4627 |
厂家: | HEWLETT-PACKARD |
描述: | Kits and Reagents for the Agilent 2100 Bioanalyzer System |
文件: | 总2页 (文件大小:217K) |
中文: | 中文翻译 | 下载: | 下载PDF数据表文档文件 |
Kits and Reagents for the
Agilent 2100 Bioanalyzer System
Agilent offers a broad range of analysis kits for the 2100 Bioanalyzer system.
•ꢀ RNAꢀqualityꢀcheckꢀ–ꢀtheꢀindustryꢀstandardꢀforꢀRNAꢀanalysis,ꢀofferingꢀtotalꢀ
RNAꢀandꢀmRNAꢀdataꢀwithꢀRNAꢀIntegrityꢀNumberꢀ(RIN),ꢀandꢀSmallꢀRNAꢀ
analysis.
•ꢀ DNAꢀsizeꢀandꢀquantityꢀ–ꢀhighꢀresolutionꢀseparationꢀandꢀquantificationꢀofꢀDNA.
•ꢀ SDS-PAGEꢀreplacementꢀforꢀProteinꢀanalysisꢀ–ꢀtheꢀfastꢀandꢀreliableꢀwayꢀtoꢀ
determineꢀtheꢀquantityꢀandꢀpurityꢀofꢀproteinsꢀfromꢀCoomassieꢀdownꢀtoꢀsilverꢀ
stainꢀsensitivity.
RNA Analysis Kits and Reagents
Order No.
Description
Order No.
Description
Order No.
Description
5067-1511ꢀ
5067-1512ꢀ
5067-1529ꢀ
RNAꢀ6000ꢀNanoꢀKit
RNAꢀ6000ꢀNanoꢀReagents
RNAꢀNanoꢀLadder
ꢀ
ꢀ
ꢀ
5067-1513ꢀ
5067-1514ꢀ
5067-1535ꢀ
RNAꢀ6000ꢀPicoꢀKit
RNAꢀ6000ꢀPicoꢀReagents
RNAꢀPicoꢀLadder
ꢀ
ꢀ
ꢀ
5067-1548ꢀ
5067-1549ꢀ
5067-1550ꢀ
SmallꢀRNAꢀKit
SmallꢀRNAꢀReagents
SmallꢀRNAꢀLadder
ꢀ
ꢀ
Analytical
RNA 6000 Nano
RNA 6000 Nano
mRNA Assay
RNA 6000 Pico
RNA 6000 Pico
mRNA Assay
Small RNA Assay
Specifications
Total RNA Assay
Total RNA Assay
ꢀ
ꢀ
Quantitativeꢀrangeꢀ
ꢀ
25–500ꢀng/µLꢀ
ꢀ
25–250ꢀng/µLꢀ
ꢀ
–ꢀ
ꢀ
–ꢀ
ꢀ
50–2000ꢀpg/µL
ofꢀpurifiedꢀmiRNAꢀinꢀwater
ꢀ
ꢀ
Qualitativeꢀrangeꢀ
ꢀ
5–500ꢀng/µLꢀ
ꢀ
25–250ꢀng/µLꢀ
ꢀ
50–5000ꢀpg/µLꢀ
inꢀwaterꢀ
250–5000ꢀpg/µLꢀ
inꢀwaterꢀ
50–2000ꢀpg/µL
ofꢀpurifiedꢀmiRNAꢀinꢀwater
ꢀ
Sizingꢀrangeꢀ
–ꢀ
–ꢀ
–ꢀ
–ꢀ
6–150ꢀnt
ꢀ
ꢀ
Sensitivityꢀ
(signal/noiseꢀ>ꢀ3)ꢀ
5ꢀng/µLꢀinꢀwaterꢀ
ꢀ
25ꢀng/µLꢀinꢀwaterꢀ
ꢀ
50ꢀpg/µLꢀinꢀwaterꢀ
200ꢀpg/µLꢀinꢀTEꢀ
250ꢀpg/µLꢀinꢀwaterꢀ
500ꢀpg/µLꢀinꢀTEꢀ
50ꢀpg/µLꢀinꢀwater**
ꢀ
ꢀ
Quantificationꢀ
reproducibilityꢀ
10%ꢀCVꢀ
(withinꢀaꢀchip)ꢀ
10%ꢀCVꢀ
(withinꢀaꢀchip)ꢀ
20%ꢀCVꢀ
(withinꢀaꢀchip)ꢀ
20%ꢀCVꢀ
(withinꢀaꢀchip)ꢀ
25%ꢀCVꢀ
(withinꢀaꢀchip)
ꢀ
Quantificationꢀaccuracy*ꢀ
20%ꢀꢀ
20%ꢀꢀ
30%ꢀꢀ
30%ꢀ
ꢀ
ꢀ
ꢀ
ꢀ
Maximumꢀsampleꢀ
100ꢀmMꢀTris,ꢀꢀ
100ꢀmMꢀTrisꢀꢀ
50ꢀmMꢀTrisꢀꢀ
50ꢀmMꢀTris,ꢀ
10ꢀmMꢀTris,
0.1ꢀmMꢀEDTA
bufferꢀstrengthꢀ
0.1ꢀmMꢀEDTAꢀ
orꢀ125ꢀmMꢀNaClꢀ
orꢀ15ꢀmMꢀMgCl2ꢀ
0.1ꢀmMꢀEDTAꢀ
orꢀ125ꢀmMꢀNaClꢀ
orꢀ15ꢀmMꢀMgCl2
0.1ꢀmMꢀEDTAꢀ
orꢀ50ꢀmMꢀNaClꢀ
orꢀ15ꢀmMꢀMgCl2
0.1ꢀmMꢀEDTAꢀ
orꢀ50ꢀmMꢀNaCl
orꢀ15ꢀmMꢀMgCl2
ꢀ
ꢀ
Physical Specifications
Analysisꢀtimeꢀꢀ
30ꢀminutesꢀꢀ
12ꢀ
30ꢀminutesꢀꢀ
12ꢀ
30ꢀminutesꢀ
11ꢀ
30ꢀminutesꢀ
11ꢀ
30ꢀminutesꢀ
11
ꢀ
ꢀ
ꢀ
ꢀ
Samplesꢀperꢀchipꢀꢀ
Sampleꢀvolumeꢀꢀ
Kitꢀstability***ꢀ
Kitꢀsizeꢀ
1ꢀµLꢀꢀ
1ꢀµLꢀꢀ
1ꢀµLꢀꢀ
1ꢀµLꢀ
1ꢀµL
4ꢀmonthsꢀ
300ꢀsamples/kitꢀ
4ꢀmonthsꢀ
300ꢀsamples/kitꢀ
4ꢀmonthsꢀ
275ꢀsamples/kitꢀ
4ꢀmonthsꢀ
275ꢀsamples/kitꢀ
4ꢀmonths
275ꢀsamples/kit
*ꢀDeterminedꢀanalyzingꢀtheꢀRNAꢀladderꢀasꢀsampleꢀ
**ꢀMeasuredꢀforꢀtheꢀ40ꢀntꢀfragmentꢀofꢀtheꢀSmallꢀRNAꢀladder
***ꢀMinimumꢀguarantee
Cell Analysis Kits and Reagents
Available only for instrument models G2938B and G2938C.
CellꢀAnalysisꢀKitꢀ(OrderꢀNo.ꢀ5067-1519)
CellꢀCheckoutꢀKitꢀ(OrderꢀNo.ꢀ5067-1520)
DNA Analysis Kits and Reagents
Order No. Description
Order No. Description
Order No. Description
Order No. Description
ꢀ
ꢀ
5067-1504ꢀ DNAꢀ1000ꢀKit
5067-1505ꢀ DNAꢀ1000ꢀReagents
ꢀ
ꢀ
5067-1506ꢀ DNAꢀ7500ꢀKit
5067-1507ꢀ DNAꢀ7500ꢀReagents
ꢀ
ꢀ
5067-1508ꢀ DNAꢀ12000ꢀKit
5067-1509ꢀ DNAꢀ12000ꢀReagents
ꢀ 5067-4626ꢀ HighꢀSensitivityꢀDNAꢀKit
ꢀ 5067-4627ꢀ HighꢀSensitivityꢀDNAꢀReagents
Analytical Specifications DNA 1000 Assay
DNA 7500 Assay
100–7500ꢀbpꢀ
DNA 12000 Assay
100–12000ꢀbpꢀ
High Sensitivity DNA Assay
50–7000ꢀbp
ꢀ
Sizingꢀrangeꢀ
25–1000ꢀbpꢀ
ꢀ
ꢀ
ꢀ
Sizingꢀresolutionꢀꢀ
25–100ꢀbp:ꢀ5ꢀbpꢀ
100–500ꢀbp:ꢀ5%ꢀ
500–1000ꢀbp:ꢀ10%ꢀ
100–1000ꢀbp:ꢀ5%ꢀ
100–1000ꢀbp:ꢀ5%ꢀ
50–600ꢀbp:ꢀ±10%
ꢀ
ꢀ
1000–7500ꢀbp:ꢀ15%ꢀ
ꢀ
1000–12000ꢀbp:ꢀ15%ꢀ
ꢀ
600–7000ꢀbp:ꢀ±20%
ꢀ
ꢀ
ꢀ
Sizingꢀaccuracy*ꢀ
10%ꢀ
10%ꢀ
15%ꢀ
10%
Sizingꢀreproducibility*ꢀꢀ
Quantificationꢀaccuracy*ꢀꢀ
5%ꢀCVꢀ
20%ꢀꢀ
5%ꢀCVꢀ
20%ꢀꢀ
5%ꢀCVꢀ
25%ꢀꢀꢀ
5%ꢀCV
20%ꢀ
ꢀ
ꢀ
Quantificationꢀreproducibility*ꢀ 25–500ꢀbp:ꢀ15%ꢀCVꢀ
100–1000ꢀbp:ꢀ10%ꢀCVꢀ
1000–7500ꢀbp:ꢀ5%ꢀCVꢀ
100–1000ꢀbp:ꢀ15%ꢀCVꢀ
50-2000ꢀbp:ꢀ15%ꢀCV
ꢀ
500-1000ꢀbp:ꢀ5%ꢀCVꢀ
0.5–50ꢀng/µLꢀ
1000–12000ꢀbp:ꢀ10%ꢀCVꢀ
2000-7000ꢀbp:ꢀ10%ꢀCV
ꢀ
Quantitativeꢀrange*ꢀꢀ
0.5–50ꢀng/µLꢀ
0.5–50ꢀng/µLꢀ
5–500ꢀpg/µL
ꢀ
ꢀ
ꢀ
Maximumꢀsaltꢀꢀ
concentrationꢀ
inꢀsampleꢀ
250ꢀmMꢀforꢀKClꢀ
250ꢀmMꢀforꢀNaClꢀ
15ꢀmMꢀforꢀMgCl2ꢀ
250ꢀmMꢀforꢀKClꢀ
250ꢀmMꢀforꢀNaClꢀ
15ꢀmMꢀforꢀMgCl2ꢀ
250ꢀmMꢀforꢀKClꢀ
250ꢀmMꢀforꢀNaClꢀ
15ꢀmMꢀforꢀMgCl2
10ꢀmMꢀTrisꢀandꢀꢀ
1ꢀmMꢀEDTA
Physical Specifications
Analysisꢀtimeꢀꢀ
ꢀ
ꢀ
ꢀ
ꢀ
ꢀ
35ꢀminutesꢀꢀ
12ꢀꢀ
30ꢀminutesꢀꢀ
12ꢀꢀ
30ꢀminutesꢀꢀ
12ꢀ
45ꢀminutes
11
Samplesꢀperꢀchipꢀꢀ
Sampleꢀvolumeꢀꢀ
Kitꢀstabilityꢀ**ꢀ
Kitꢀsizeꢀ
1ꢀµLꢀꢀ
1ꢀµLꢀꢀ
1ꢀµLꢀ
1ꢀµL
4ꢀmonthsꢀ
300ꢀsamples/kitꢀ
4ꢀmonthsꢀ
300ꢀsamples/kitꢀ
4ꢀmonthsꢀ
300ꢀsamples/kitꢀ
4ꢀmonthsꢀꢀ
110ꢀsamples/kit
*ꢀDeterminedꢀanalyzingꢀtheꢀDNAꢀladderꢀasꢀsample
**ꢀMinimumꢀguarantee
Protein Analysis Kits and Reagents
Order No. Description
Order No. Description
Order No. Description
ꢀ
ꢀ
5067-1515ꢀ Proteinꢀ80ꢀKit
5067-1516ꢀ Proteinꢀ80ꢀReagents
ꢀ
ꢀ
5067-1517ꢀ Proteinꢀ230ꢀKit
5067-1518ꢀ Proteinꢀ230ꢀReagents
ꢀ 5067-1575ꢀ HighꢀSensitivityꢀProteinꢀ250ꢀKit
ꢀ 5067-1576ꢀ HighꢀSensitivityꢀProteinꢀ250ꢀReagents
ꢀ 5067-1577ꢀ HighꢀSensitivityꢀProteinꢀ250ꢀLabelingꢀReagents
ꢀ 5067-1578ꢀ HighꢀSensitivityꢀProteinꢀ250ꢀLadder
Analytical Specifications Protein 80 Assay
Protein 230 Assay
14–230ꢀkDaꢀ
High Sensitivity Protein 250 Assay
10–250ꢀkDa
ꢀ
ꢀ
ꢀ
ꢀ
Sizingꢀrangeꢀ
5–80ꢀkDaꢀ
Typicalꢀsizingꢀresolutionꢀ
Typicalꢀsizingꢀaccuracyꢀ
Sizingꢀreproducibilityꢀ
10%ꢀ
10%ꢀ
10%
10%ꢀ(CAII,ꢀBLG)ꢀ
3%ꢀCVꢀ(CAII,ꢀBLG)ꢀ
10%ꢀ(BSA,ꢀCAII)ꢀ
3%ꢀCVꢀ(BSA,ꢀCAII)ꢀ
10%ꢀ(BSA)
3%ꢀCVꢀ(BSA)
ꢀ
ꢀ
ꢀ
Sensitivityꢀ(signal/noiseꢀ>ꢀ3)ꢀ
6ꢀng/µLꢀCAIIꢀ(15ꢀng/µLꢀBSA)ꢀinꢀPBSꢀ 6ꢀng/µLꢀCAIIꢀ(15ꢀng/µLꢀBSA)ꢀinꢀPBSꢀ
1ꢀpg/µLꢀ(labeledꢀBSA)ꢀinꢀwaterꢀonꢀchip
5ꢀpg/µLꢀ(labeledꢀBSA)ꢀinꢀPBSꢀonꢀchip
Labelingꢀreactionꢀatꢀ1ꢀng/µLꢀofꢀtotalꢀprotein
ꢀ
ꢀ
10ꢀng/µLꢀ(CAII)ꢀinꢀ0.5ꢀMꢀNaClꢀꢀ
(30ꢀng/µLꢀBSAꢀinꢀ0.5ꢀMꢀNaCl)ꢀ
30ꢀng/µLꢀ(BSA)ꢀinꢀ0.5ꢀMꢀNaClꢀ
ꢀ
ꢀ
ꢀ
Quantitativeꢀrangeꢀ
ꢀ
60–2000ꢀng/µLꢀCAIIꢀinꢀPBSꢀ
ꢀ
15–2000ꢀng/µLꢀCAII,ꢀ
Upꢀtoꢀ4ꢀordersꢀofꢀmagnitudeꢀ
(0.3ꢀtoꢀ3000ꢀng/µLꢀBSA)
30–2000ꢀng/µLꢀBSAꢀinꢀPBSꢀ
ꢀ
ꢀ
Qualitativeꢀrangeꢀ
ꢀ
6–4000ꢀng/µLꢀCAIIꢀandꢀBLGꢀ
ꢀ
6–5000ꢀng/µLꢀCAII,ꢀꢀ
–
15–5000ꢀng/µLꢀBSAꢀinꢀPBS
ꢀ
Quantificationꢀreproducibilityꢀ
20%ꢀCVꢀ(CAII,ꢀBLG)ꢀꢀ
20%ꢀCVꢀ(BSA,ꢀCAII)ꢀ
20%ꢀCVꢀ(BSA)
Physical Specifications
ꢀ
ꢀ
ꢀ
ꢀ
ꢀ
Analysisꢀtimeꢀꢀ
Samplesꢀperꢀchipꢀꢀ
Sampleꢀvolumeꢀꢀ
Kitꢀstabilityꢀ*ꢀ
Kitꢀsizeꢀ
30ꢀminutesꢀꢀ
10ꢀꢀ
25ꢀminutesꢀ
10ꢀ
30ꢀminutes
10
4ꢀµLꢀꢀ
4ꢀµLꢀ
5ꢀµL
4ꢀmonthsꢀꢀ
250ꢀsamples/kitꢀ
4ꢀmonthsꢀ
250ꢀsamples/kitꢀ
6ꢀmonths
100ꢀsamples/kit
CAIIꢀ=ꢀCarbonicꢀAnhydrase,ꢀBSAꢀ=ꢀBovineꢀSerumꢀAlbumin,ꢀBLGꢀ=ꢀbeta-Lactoglobulin
*ꢀMinimumꢀguaranteeꢀ
www.agilent.com/genomics/bioanalyzer
ForꢀResearchꢀUseꢀOnly.
Notꢀforꢀuseꢀinꢀdiagnosticꢀprocedures.
Thisꢀinformationꢀisꢀsubjectꢀtoꢀchangeꢀwithoutꢀnotice.
©ꢀAgilentꢀTechnologies,ꢀInc.ꢀ2017
PublishedꢀinꢀUSA,ꢀFebruaryꢀ1,ꢀ2017
5991-7887EN
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